Abstract
The 1H NMR spectrum of ampicillin in aqueous solution at pD 8 and 4 was assigned using a combination of 2D T-ROESY and 1D GOESY techniques. The Me-α and Me-β chemical shift assignments, determined at pD 8, are opposite to existing literature values obtained under similar conditions. Additionally, the methyl chemical shifts were found to be strongly pD dependent, with a chemical shift crossover occurring at lower pD. Copyright © 2000 John Wiley & Sons, Ltd.
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