On the use of partition coefficients to characterize the distribution of fluorescent membrane probes between coexisting gel and fluid lipid phases: an analysis of the partition behavior of 1,6-diphenyl-1,3,5-hexatriene

Abstract

The distribution of the fluorescent membrane probe 1,6-diphenyl-1,3,5-hexatriene between coexisting gel and fluid phospholipid phases in multilamellar vesicles has been examined using fluorescence quenching by spin-labeled phosphatidylcholine. For both thermally-induced and Ca 2+-induced lipid phase separation, the ratio of probe concentration in the fluid liquid-crystal phase to that in the gel phase is found to be independent of either the probe concentration or the relative amounts of gel and fluid lipid phases, and hence is an equilibrium concentration ratio, or partition coefficient.