Abstract
The effect of pH, ionic strength, urea and organic solvents on the formation of precipitates between a charged antigen, p(Glu, Tyr)-p dlAla—pLys, an uncharged antigen, des-p(hydroxypropylGlu, Tyr)-p dlAla—pLys, and their homologous antibodies was investigated. The pH and ionic strength effects were similar in each case, implying that electrostatic forces are not uniquely responsible for antigen-antibody interactions. Addition of dioxane, dimethylformamide or urea almost completely prevented the formation of precipitates in the uncharged antigen system, while it influenced the charged antigen system much less. It is suggested that hydrophobic forces are of importance in the reaction of antigen with antibody, and that, when present, charged groups contribute to the stabilization of the antigen-antibody interaction.
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