Abstract
1. 1. The lipids of liver were reexamined for polyphosphoinositide, previously reported absent. The presence of a diphosphoinositide (DPI) was revealed by the isolation of inositol diphosphate (IP 2) from an acid hydrolysate, and its glyceryl derivative from an alkaline hydrolysate of the concentrated phospholipid fraction. 2. 2. Starting with lipids extracted from liver and pancreas by azeotropic dehydration with 1,2-dichloroethane, materials soluble in acetone and alcohol were removed. After partition of the alcohol-insoluble phospholipids in CCl 4-light petroleum-methanol-H 2O (31:31:35:3, V/V), the non-polar fraction was assayed for inositol polyphosphate by chromatographic fractionation of the acid hydrolysate on Dowex-2 X8 Cl − A diphosphate mixture eluted by NaCl or LiCl was separated by refractionation with a formate system yielding IP 2 and glycerol diphosphate. 3. 3. The mixed glyceryl derivatives of IP 2 and glycerol diphosphate were secured by chromatographic fractionation of the alkaline hydrolysate. Based on the recovery of IP 2 and its glyceryl derivative, the content of DPI in the original pork liver lipid is 0.28 mmoles/kg. 4. 4. DPI could not be extracted by ether from acetone-dehydrated fresh beef liver but was extracted with neutral chloroform-methanol (2:1, V/V). 5. 5. Most of the IP 2 found in pacreatic lipid occured in material insoluble in CHCl 3 and in the CCl 4-light petroleum solvent.
Published Version
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