Abstract
Polynucleotide-joining enzymes (ligases) have been identified in uninfected and phage-infected Escherichia coli (Gellert, 1967; Olivera and Lehman, 1967a; Zimmerman et al., 1967; Gefter et al., 1967; Weiss and Richardson, 1967a; Cozzarelli et al., 1967). Both types of enzyme catalyze the synthesis of a phosphodiester bond between the 3′ hydroxyl and 5′ phosphoryl termini of DNA chains which have been properly aligned in a double-helical structure. Such enzymes are of interest because of their possible involvement in genetic recombination, ‘dark’ repair of ultraviolet-induced lesions, and in DNA synthesis. It has already been demonstrated that the polynucleotide ligase induced by phage T4 is essential for normal T4 DNA replication (Fareed and Richardson, 1967).
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call