On the evaluation of the pregnancy test by the occurrence of polyamine in the bovine blood plasma

Abstract

Morgantini's pregnacy diagnosis test, the colour reaction of blood plasma produced by heating in the presence of cupric carbonate, was performed with bovine blood plasma in various conditions. But the availability of the test resulted in definitely negative since results of the reaction happened to be almost all positive regardless to the pregnancy or not.Next, spermine, which regarded as the reactive substance in the preceding colour reaction, was detected together with other amines in blood plasma by paper-chromatography to know whether the amines appeared specifically in pregnant plasma. Volatile amine fraction was prepared from the TCA soluble fraction by the vapour distillation with Kjeldahle apparatus. The findings by the paper-chromatography showed that two spots, corresponded to spermine or spermidine and putrescine, also existed independent of the animal condition either pregnancy or lactation. Therefore, the authors completely failed to ascertain the Morgantini's investigation as the pregnancy diagnosis test based on either the colour reaction with cupric carbonate or the detection of spermine with paper-chromatography in bovine plasma. The occurrence of polyamines in the bovine blood plasma and the pregnancy tests which seemed to be related to this test was briefly discussed.