On the Epinephrine Estimates Obtainable by Some Methods, Chemical and Biological, in the Extract of Bovine Suprarenal Medulla

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Medullary tissue was isolated from fresh bovine suprarenal gland, frozen by means of liquid nitrogen, and made further as homogenous powder. From its portion the extract was made according to Folin, Cannon and Denis's procedure, and from another according to Suto. Of the former, epinephrine was estimated by some colorimetrial methods, such as the persulphate method, the permanganate method, the iodine method, and the phosphotungustate method (Folin) and the mercuric and some biological methods such as the cat paradoxical pupil method, the blood pressure method and the rabbit intestine segment method. Of the latter extract epinephrine was determined by the Suto-Kojima. Adrenaline hydrochloride of Sankyo Co., assayed by means of Folin-Kodama's method, was used as the standard. Four colorimetrical methods yielded similar figures, Folin's method having given a little greater value. The cat paradoxical pupil method gave a little, but definitely greater value than the color-imetrical; the blood pressure method still greater, and the rabbit intestine method the greatest. If the mean value yielded by the persul-phate method be taken as 1, the remainder gives the following: The permanganate 1.02, the iodine 1.07, Suto 1.07; the Folin 1.14. The paradoxical pupil 1.24, the blood-pressure 1.57 and the Intestine 1.84. These figures at least indicate with definiteness as to it being unnatural to assume that two kinds of estimating, colorimetrical and biological, yield an equal value of epinephrine for bovine suprarenal medulla.