On the determination of adrenaline in suprarenal extracts by some chemical and biological methods.

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The whole figure of the present data will be given as, a whole once in the tabular form, Tab. VI, and again briefly in the following paragraphs. The medulla of cattle, horses and dogs involves the adrenaline, determinable by the chemical methods, as the Folin, the Suto, the Stuber and the Shaw, in about 1% of tissue and that by the rabbit intestine seg-ment method in about 1.5%. But, exactly examined, the values obtained by these chemical methods not only do not check with each other, but there are also definite differences not negligible. And they are different accord-ing to the animal species, as readily seen in the summary table. On the whole, while the Suto method yields commonly the smallest .amount of adrenaline, or always a smaller amount than the Folin, the: Stuber and the rabbit intestine segment, the Shaw method gives to the bovine and horse suprarenal medullary tissue a similar value to the Suto, but to the dog medullarly tissue and rabbit suprarenal a definitely smaller -one than the Suto method. Notwithstanding that the Suto method and the Stuber are similarly the reaction with mercuric chloride, and they give quite the same amount of adrenaline to the dog's suprarenal medulla and to the rabbit suprarenals, they give on the other hand dissimilar value for the bovine and horse medullary tissues, such as 11% by the Stuber and 10% by the Suto for the bovine tissue and 11.5% by the Stuber and 10.5% by the Suto for the horse tissue. Briefly recapitulated: The methods of Stuber et al and of Shaw have been further added in solving the problem how the active principles in the suprarenal medulla, which acts as adrenaline or epinephrine, both physio-logically and chemically, are to be appreciated duly. They gave us then further evidences for our conviction that it is not legitimate to assume -only an active principle such as the adrenaline or epinephrine exists solely there, but the matter is of much complex nature.