Abstract
The protease inhibitor (Pi) types in light-breed horses were studied by isoelectric focusing (IEF) and two-dimensional (2-D) electrophoresis. Since equine Pi types are highly heterogeneous, establishment of confirmatory techniques for correctly distinguishing each Pi type has been an important issue, and 2-D electrophoresis was found to be effective for this. Each Pi type with PiS and PiT alleles was subjected to 2-D electrophoresis, and their characteristics were clearly shown in photographic form.
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