Abstract
Isoelectric focusing (IEF) using polyacrylamide gel containing urea (Urea-PAG) was used for the characterization of equine protease inhibitor (Pi) types in light breed horses to solve the following problems in conventional IEF and two-dimensional (2-D) electrophoresis: typing complexity due to high heterogeneity of Pi system and overlapping of Pi and esterase (Es) components on IEF, operational complexity and time-consumption on 2-D. The Urea-PAG IEF method had high resolution capacity, clearly distinguishing at least 10 types frequently observed in light breed horses. Es components were not detected on Urea-PAG. There was little complexity of Pi typing as shown by these results, and thus the typing procedure is simpler and more rapid than conventional IEF and 2-D. The present method should thus prove quite adequate for exact classification of Pi system in light breed horses.
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