Abstract
A direct method for the determination of piroxicam in plasma is described. Plasma is directly injected onto the extraction column (10 mm × 2 mm I.D., packed with 40-μm Bond Elut C 2) where piroxicam is separated from the plasma concomitants using a solid-phase extraction procedure. Using a laboratory-made on-line column-switching system, the drug is quantitatively transferred and separated on the analytical column (15 cm × 4.6 mm I.D., Supelcosil LC18 DB, 5 μm) followed by determination using ultraviolet absorption at 331 nm. Validation of the method demonstrated a good recovery (100%), sensitivity (limit of determination 0.2 μg/ml, based on a 20-μl sample volume), accuracy and precision (better than 5%). The developed method has been adopted for studying the steady-state pharmacokinetics of the drug.
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