Abstract
We present an on-demand single cell imaging technique activated by differential resistive pulse sensing in a portable system integrating a microfluidic differential coulter counter and a lensless complementary metal-oxide-semiconductor (CMOS) imaging sensor. Dual parametric single cell analysis and on-demand single cell imaging have been demonstrated by microbeads of different sizes and a cell mixture including red blood cells (RBCs) and tumor cell line HepG2 cells. The on-demand imaging capability could avoid generating useless images without cells and enable selective imaging of single cells within a specific size range.
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