Abstract
A method for the on-column preparation of chemically bonded stationary phase with monofunctional reagents was studied in conventional stainless-steel columns and in glass capillary columns for packed microcapillary chromatography. Simple reactions of the silica surface with octadecyldimethyl-N,N-diethylaminosilane in packed columns resulted in a surface coverage of more than 3.0 μmol/m 2, comparable to the stationary phase of maximum coverage prepared in batch. The extent of surface coverage and the amount of residual silanols were carefully examined by using positively charged amines and neutral compounds. Although the results indicate a slight difference between the C 18 phases in stainless-steel columns and in packed microcapillary columns, excellent reproducibility was found for the on-column bonding reaction, as expected for the reaction using monofunctional reagents.
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