Abstract
This work reports the synthesis of a novel copolymer, poly(AA-PBA-PAA) via an easy one-pot method, the fabrication of boronic acid containing surface layer on silicon chip, and the sandwich immunoassay using magnetic nanoparticles (MNPs) as signal label for fast determination of HbA1c. Poly(AA-PBA-PAA) was synthesized with acrylic acid (AA), 3-aminophenylboronic acid (APBA) and 4-Aminobenzylamine (APAA) by EDC/NHS amidation and RAFT. The molecule structure and molecule weight of the poly(AA-PBA-PAA) were characterized by nuclear magnetic resonance spectroscopy (1H NMR), fourier transform infrared spectroscopy (FT-IR) and gel permeation chromatography (GPC). The presence of aniline residues allowed the copolymer covalently immobilizing on polystyrene grafted maleic anhydride (PS-MA) coating. We also confirmed the sandwich structure of boronic acid affinity immunoreaction spot on optical microscope and optical profiler. By the means of magnetic nanoparticles counting, a linear range between 0.1–10μgmL−1 was achieved for the determination of HbA1c. This strategy possesses high sensitivity with detection limit of 17.7ngmL−1 and excellent stability at 4°C. The repeatability is evaluated with CV values less than 5% for 10 magnetic nanoparticle number calibrations on the same chip, and less than 10% for 10 different batches. Finally, a comparison of THb and HbA1c determination with different eluents demonstrates significant specificity of this method.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call