Сomplex Phototherapy for Compensation of Damages Induced by High-Intensity Laser Radiation in Experiment

Abstract

The aim of the investigation was to develop a new technology of complex phototherapy based on consecutive biological tissue exposure to high-intensity laser radiation and low-intensity broadband red light and to evaluate its possibilities in experiment. Materials and Methods. There were studied the effects of consecutive exposure of white rat tissues to high-intensity red and infrared radiation and low-intensity broadband red light on spontaneous and metal-catalyzed oxidative modification of proteins. The study was performed on white outbred rats with a body mass of 180 to 250 g, which were divided into 5 groups. Control group 1 included animals whose inner surface of the thigh was exposed locally to laser light radiation with a wavelength of 671 nm (red laser) and the power of 50 mW. Test group 2 underwent locally red laser radiation of the inner femoral surface and three consecutive sessions of low-intensity broadband red light exposure (a wavelength of 630 nm, light spot intensity of 5 mW/cm 2 ). Control group 3 was exposed locally to 980 nm laser radiation of the inner femoral surface (infrared laser with the power of 50 mW). Test group 4 was exposed locally to infrared laser radiation of the inner femoral surface and three consecutive sessions of low-intensity broadband red light. Group 5 (intact) was not exposed to radiation. Samples of femoral tissue and blood serum were taken on day 3 in all groups of animals. Results. The content of neutral and basic aliphatic dinitrophenylhydrazons in the tissues exposed to red and infrared laser radiation was found to be increasing. in subsequent sessions of low-intensity red light phototherapy there occurred sharp decrease in the levels of protein oxidative modification products to normal values. Conclusion. The developed technology of complex phototherapy including sessions of consecutive exposure of biological tissues to laser and broadband red light provides radiation safety of procedures.