Abstract
Exposure of thymine and DNA to high-intensity 532 nm pulsed radiation from a Nd:YAG laser resulted in the cyclobutylpyrimidine dimers, which were measured by the method of high performance liquid chromatography. The in vitro transcription by RNA polymerase was markedly inhibited and could not be stimulated by spermine when the native double-strand DNA was replaced by irradiated DNA. It was shown that DNA damage was caused by 532 nm laser radiation and that the high-intensity visible radiation can initiate photochemistry in UV-absorbing biological molecules by two photon absorption. It is suggested that the use of very high-intensity laser radiation in medicine introduces the possibility that biomacromolecules may be damaged in cells as a result of two photon absorption.
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