Abstract
The use of separation techniques such as gas, liquid and thin-layer chromatography as well as capillary electrophoresis for metabonomics/metabolomics is described. Capillary gas chromatography (GC), especially when linked to mass spectrometric detection provides a very powerful means of metabolite profiling. This is particularly the case when GC X GC is performed. However, the GC analysis of complex biological samples requires extensive sample pre-treatment and derivatization prior to analysis and chromatography is generally slow. High performance liquid chromotagraphy (HPLC), capillary LC and ultra performance liquid chromatography (UPLC) are capable of providing high throughput, generally with little sample preparation (other than protein precipitaion/removal of particulates) required. Capillary electrophoresis (CE) is another high-resolution liquid phase technique that complements those of liquid chromatography because of the alternative separation mechanism involved. Thin-layer chromatography (TLC) has potential in metabonomics/metabonomic analysis but has not been widely used to date.
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