The use of liquid and gas chromatography in combination with mass spectroscopy and the method of capillary electrophoresis to study hydroxycinnamic acids in plants growing in Russia

Abstract

BACKGROUND: Recently, the volume of research and publications devoted to the study of the class of phenylpropanoids in plants, in particular, hydroxycinnamic acids and their derivatives, has been steadily growing. This is due to the diverse range of their pharmacological activity and the possibility of using them in medicine. The reliability and reliability of identification of this group of compounds is significantly expanding with the improvement of analytical methods: gas and liquid chromatography in combination with mass spectroscopy, as well as the use of capillary electrophoresis. The study of the proposed approaches to the analysis of this group of compounds can be interpolated to new plant objects. AIM: A comparative analysis of the use of liquid and gas chromatography methods in combination with mass spectroscopy, as well as the capillary electrophoresis method for the study of hydroxycinnamic acids in plants growing and cultivated in the Russian Federation. MATERIALS AND METHODS: Results published in domestic periodical scientific journals and conference proceedings, based on information and analytical studies. RESULTS: Data on the study of the conditions of extraction, structure and content of hydroxycinnamic acids and their derivatives in plants growing on the territory of the Russian Federation have been systematized. The review presents the advantages and limitations of gas and liquid chromatography in combination with the method of mass spectroscopy in the analysis of this group of compounds, using various conditions for their extraction from plant materials. The prospects of using the capillary electrophoresis method for these purposes are shown due to the ease of implementation and highly efficient separation of hydroxycinnamic acids and their derivatives in plant materials. It has been established that there is a lack of information on studying the dynamics of their accumulation depending on climate-forming factors, regions of growth, as well as their stability in plant materials. CONCLUSION: analysis of the methods presented in the review allows us to create a methodological basis for further improvement and development of new methods for the analysis of hydroxycinnamic acids and their derivatives in plant objects.