Oily bilge water treatment using indigenous soil bacteria: Implications for recycling the treated sludge in vegetable farming

Abstract

Hydrocarbon contamination from motorized vessels operating on seas threaten marine ecosystems and need to treated efficiently. A bilge wastewater treatment using indigenous bacteria isolated from oil contaminated soil was studied. Five bacterial isolates (Acinetobacter baumanni, Klebsiella aerogenes, Pseudomonas fluorescence, Bacillus subtilis and Brevibacterium linens) were isolated from port soil and used in the bilge water treatment. Their crude oil degradation abilities were first confirmed experimentally. The single species and the consortia of each two species were compared in an experiment where the conditions were first optimized. The optimized conditions were 40 °C, carbon source glucose, nitrogen source ammonium chloride, pH 8, and salinity 25%. Each of the species and each combination was able to degrade oil. K. aerogenes and P. fluorescence were the most efficient in reducing the crude oil concentration. The crude oil concentration was reduced from 290 mg/L to 23 mg/L and 21 mg/L, respectively. The respective values for the loss in turbidity were from 320 NTU to 29 mg/L and 27 NTU and for BOD loss from 210 mg/L to 18 mg/L and 16 mg/L. Mn was reduced from 25.4 mg/L to 1.2 mg/L and 1.0 mg/L, Cu from 26.8 mg/L to 2.9 mg/L and 2.4 mg/L, and Pb from 29.8 mg/L to 1.5 mg/L and 1.8 mg/L. The consortium of K. aerogenes and P. fluorescence in the bilge wastewater treatment reduced the crude oil concentration to 11 mg/L. After the treatment, the water was removed and the sludge was composted with palm molasses and cow dung. After 60 days of composting and inoculation with different bacterial consortia, the final product was used as a seedbed for vegetables. The compost with the consortium K. aerogenes and P. fluorescence promoted vegetable plant growth most and could be used in farming.