Abstract
Essential oils (EOs) are volatile, natural, complex compounds characterized by a strong odour and are formed by aromatic plants as secondary metabolites. The essential oil of Lavendula officinalis was extracted using hydrodistillation process. Hydro-Distillation is potentially a very useful method to extract essential oil from various plants and from their different parts. The principle of extraction is based on the isotropic distillation. The yield is dependent on various parameters like weight of raw material, volume of water, size of raw material and nature of raw material. Samples from aerial parts of three Lavendula ecotypes were analyzed for essential oil content to find out as to which ecotype excels in oil content and which ecotype gave the minimum essential oil output. Air dried leafy stalks and stems of L. officinalis were taken and submitted to Hydro distillation for 4 hrs using Clevenger type apparatus. Briefly, the samples were immersed in water and heated to boiling, after which the essential oil was evaporated together with water vapour and finally collected in a condenser. The distillates (EOs) were isolated and dried over anhydrous sodium sulphate. The oils were stored in the sealed vials at 2°C for further studies. Each extraction was performed at least three times. The oil content was determined on an oil volume to tissue weight basis. Estimated oil content was lowest in Srinagar ecotype to a highest in Pulwama ecotype. Pulwama ecotype was followed by Budgam ecotype. This could be due to the variation in altitude and location.
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