Abstract
The aim. Study of oil oxidative activity of some strains of bacteria of Pseudomonas genus, selected for use in bioremediation of soil. Methods. Bacteria P. fluorescens ОNU328, P. maltophilia ОNU329, P. cepacia ОNU327 have been cultivated in nutrient medium M-9 contained crude oil with density of 0.84 g /cm3 with the concentration 500 mg /l. The residual content of oil in 10, 20 and 30 days, was determined by the integral method of infrared spectroscopy. Oil extraction of the samples was performed by using carbon tetrachloride (CCl4). Results. It was determined that the bacteria of genus Pseudomonas selected for use in biotechnology of purification of soil, within a month broke down hydrocarbons of crude oil with initial concentration of 500 mg /dm3 up to 70–80%. Oil oxidative activity of all used strains of microorganisms was high in the first 10 days of the exhibition – the degree of biodegradation of petroleum hydrocarbons reached 62.0–73.0%. Conclusion. Bacteria strains of P. cepacia ОNU327, P. maltophilia ONU329 and P. fluorescens ОNU328 can be recommended for the development of technologies of soil bioremediation from oil and oil products.
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