Odorant-binding proteins involved in sex pheromone and host-plant recognition of the sugarcane borer Chilo infuscatellus (Lepidoptera: Crambidae).

Abstract

Pheromone-binding proteins (PBPs) are responsible for transporting sex pheromones and general odorant-binding proteins (GOBPs) have been proposed to transport host-plant volatiles. A large number of OBPs have been identified from Lepidoptera species. However, olfactory molecular biology and physiology studies on PBP and GOBP in sugarcane pests are limited. Chilo infuscatellus is one of the most widely distributed pests in sugarcane-producing areas. Three PBPs (CinfPBP1, CinfPBP2 and CinfPBP3) and two GOBPs (CinfGOBP1 and CinfGOBP2) were identified, and five olfactory gene transcripts were abundantly expressed in antennae of C. infuscatellus. Binding assays showed that CinfPBP1-3 exhibited strong binding affinity for the sex pheromone components Z11-16:OH and 16:OH of C. infuscatellus. Meanwhile, CinfGOBP1-2 had high binding affinity with host-plant volatiles from sugarcane (Saccharum officinarum). Field-trapping results suggested that four volatile components, octadecane, (Z)-3-hexen-1-ol, α-terpineol and hexadecane from host plants and sex pheromone mixed baits have synergistic roles in attracting C. infuscatellus adult moths. Functional characterization of CinfPBPs and CinfGOBPs in C. infuscatellus could help us find new environmentally friendly alternatives to conventional pest control using pesticides in sugarcane fields. © 2020 Society of Chemical Industry.