Molecular characterization and functional analysis of pheromone binding proteins and general odorant binding proteins from Carposina sasakii Matsumura (Lepidoptera: Carposinidae).

Abstract

The peach fruit moth, Carposina sasakii Matsumura (Lepidoptera: Carposinidae), is one of the most destructive pests of pome and stone fruits, while few studies of their molecular biology and physiology have been conducted. Research into CsasPBPs (Carposina sasakii pheromone binding proteins) and CsasGOBPs (Carposina sasakii general odorant binding proteins) may provide insights in to the mechanisms of olfaction in Carposina sasakii. In our study, results of real time quantitative polymerase chain reaction (qPCR) assays demonstrated that CsasPBP1-3 and CsasGOBP1-2 transcripts were abundantly expressed in the antennae of both sexes, suggesting they play a vital role in olfaction. In addition, to examine specific functional differences between pheromone binding proteins (PBPs) and general odorant binding proteins (GOBPs), fluorescence competitive binding assays were used to measured the binding affinities for the two sex pheromones and 18 apple plant volatiles. As a result, both PBPs and GOBPs showed stronger binding affinities to Z-7-eicosene-11-one than Z-7-nonadecene-11-one in two sex pheromones, whereas only PBP3 exhibited specific affinity towards both these two sex pheromone components, and PBP1 showed a high binding affinity to the sex pheromone components, and to other plant volatiles. In addition, GOBP1-2 displayed high binding affinity to general components of plant volatiles. Our study suggested CsasPBPs and CsasGOBPs play distinct physiological roles in the perception of sex pheromones and host plant volatiles. © 2018 Society of Chemical Industry.