Abstract
<p><strong>Background.</strong> Tomato brown rugose fruit virus (ToBRFV) is an emerging pathogen distributed in different regions of Mexico. <strong>Objective.</strong> To test the presence of ToBRFV in tomato plants of the Mesías variety (HM-Clause) in five greenhouses located in San Pablo Guilá, Oaxaca. <strong>Methodology.</strong> Using ImmunoStrip (Agdia, Elkhart, USA) a serological assay was performed on the five samples collected from the greenhouse. Using a modified version of Koch's postulates, tomato plants var.192-536 F1 were mechanically inoculated. A biological indexing assay was also performed, where 30-day-old plants of <em>Chenopodium amaranticolor</em> and <em>Nicotiana longiflora</em> were inoculated from infective sap prepared from each sample collected. The sandwich ELISA (DAS-ELISA) was performed 30 days post inoculation (dpi). Subsequently, a fragment of the 475 bp region was amplified by reverse transcription (RT) and polymerase chain reaction (PCR). <strong>Results.</strong> The ImmunoStrip assay was positive for ToBRFV, inoculation of plants 30 dpi, expressed symptoms of blistering, yellowing, mosaic and leaf deformation. <em>N. longiflora</em> plants showed local necrotic lesions, while <em>Ch. amaranticolor</em> expressed the formation of local chlorotic lesions. Likewise, the samples run by DAS-ELISA yielded a positive diagnosis for ToBRFV. The RT-PCR products obtained amplified a 475 bp fragment confirming the presence of ToBRFV in the five samples studied. <strong>Implications.</strong> There are limited reports confirming the presence of ToBRFV in Mexican states. <strong>Conclusions.</strong> To our knowledge, this is the first occurrence of ToBRFV infecting tomato plants in greenhouses in Oaxaca, Mexico.</p>
Published Version
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