Ocean acidification induces tissue-specific interactions with copper toxicity on antioxidant defences in viscera and gills of Asiatic hard clam Meretrix petechialis (Lamarck, 1818)

Abstract

Toxicity of contaminants in organisms under ocean acidification (OA) has attracted increasing attention in ecotoxicological studies. This study investigated how pCO2-driven OA affected waterborne copper (Cu) toxicity in antioxidant defences in viscera and gills of Asiatic hard clam Meretrix petechialis (Lamarck, 1818). Clams were continuously exposed to Cu at ambient relevant (0/no metal exposure, 10 and 50 μg L−1) and polluted-high (100 μg L−1) concentrations in unacidified (pH 8.10) and acidified (pH 7.70/moderate OA and 7.30/extreme OA) seawater for 21 days. Following coexposure, metal bioaccumulation and responses of antioxidant defence-related biomarkers to OA and Cu coexposure were investigated. Results showed that metal bioaccumulation was positively correlated with waterborne metal concentrations but was not notably influenced by OA conditions. Both Cu and OA affected the antioxidant responses to environmental stress. Additionally, OA induced tissue-specific interactions with Cu on antioxidant defences, varying with exposure conditions. In unacidified seawater, antioxidant biomarkers were activated to defend against oxidative stress induced by Cu and prevented clams from lipid peroxidation (LPO or MDA), but failed to defend against DNA damage (8-OHdG). OA exacerbated Cu toxicity in antioxidant defences and increased LPO levels in tissues. Gills and viscera adopted adaptive antioxidant defence strategies to manage oxidative stress, with the former being more vulnerable to oxidative stress than the latter. MDA and 8-OHdG were sensitive to OA and Cu exposure, respectively, and were useful bioindicators for assessing oxidative stress. Integrated biomarker response (IBR) and PCA can reflect the integrative responses of antioxidant biomarkers to environmental stress and illuminate the contributions of specific biomarkers to antioxidant defence strategies. The findings provided insights for understanding antioxidant defences against metal toxicity in marine bivalves under OA scenarios, which is essential into managing wild populations.