Occurrence of larval and adult types of ion-secreting ionocytes in Japanese eel Anguilla japonica

Abstract

The anguillid eels are catadromous fishes that migrate between marine and freshwater habitats. The long migration of eel larvae, called leptocephali, as long as thousands of kilometers in the ocean is important to determine their recruitment successes. The leptocephali in the ocean have a pelagic lifestyle totally different from the benthic one of glass eels and yellow eels in rivers. It is known that eel leptocephali have ionocytes on the body surface that may maintain ionic and osmotic status in the internal environment; however, detailed morphology and function of ionocytes in leptocephali are still unknown. In the present study, we aimed 1) to clarify the morphological features of the epidermis in Japanese eel Anguilla japonica leptocephali cultured in hyper-osmotic condition, and 2) to examine the ion-transporting functions of ionocytes of both leptocephali and yellow eels. Na+/K+-ATPase-immunoreactive ionocytes were distributed all over the body surface of leptocephali. Ionocytes were in contact with external environments through their apical membrane, which was located at the boundary of pavement cells. Na+/K+-ATPase-immunopositive cells were not observed in the skin of seawater-acclimated yellow eels. In ionocytes of the larval skin, the apical membrane appeared as a slightly projecting disk with a microvilli-like structure. Meanwhile, the apical membrane of gill ionocytes of yellow eels formed a concave surface. In ionocytes of leptocephali, mitochondria were enlarged and the tubular system was well developed, as compared with those of the gill of yellow eels. Ionocytes of leptocephali showed CFTR immunoreaction in their apical region and NKCC1 immunoreaction in their basolateral region, suggesting that the skin ionocytes are involved in salt secretion. These results support the notion that Japanese eel maintain their ion balance through skin ionocytes during early life stages, and that the skin ionocytes of leptocephali disappear in yellow eel stages after the formation of functional gills and gill ionocytes.