Occurrence of a novel tannase (tan BLP ) in endophytic Streptomyces sp. AL1L from the leaf of Ailanthus excelsa Roxb.

Abstract

The tannase production ability by endophytic actinobacteria and the genetic identity of responsible tannase gene were determined. The studied strains were isolated from surface-sterilized leaf discs of Ailanthus excelsa Roxb. Four strains were found to hydrolyze tannic acid on solid media containing 0.4% tannic acid. The strain AL1L was found as tanBLP indicating production of tannase with diverse of substrate affinity. The tannase production from the potential strain AL1L was performed in liquid tannic acid broth (0.4%, w/v). The strain was later identified as Streptomyces sp. AL1L on the basis of 16S rDNA homology. Highest enzyme activity was observed at 48h of incubation at the exponential growth phase. The enzyme was purified by ammonium sulfate precipitation followed by dialysis (15 kD cut off). This enzyme, with molecular weight 180 kD shows highest catalytic activity at 35°C, pH 6 with substrate concentration 0.1g%. The purified enzyme possesses 1.4×10-3Km and 11.15 U/ml as Vmax. The above study indicates high industrial prospective of endophytic actinobacteria as source of tannase of potential biotechnological applications.