Manipulation of fermentation conditions on production of Tannase from agricultural by-products with Aspergillus oryzae

Abstract

Tannin acyl hydrolase is an industrially important enzyme that is mainly used in the food and pharmaceutical industry. As the range of applications of this enzyme is very wide, there is always a scope for novel Tannase with better characteristics, which may be suitable in the diverse fields of applications. The present work has been taken up with a view of exploring the possibilities of using agricultural by products as a source for the production of Tannase and optimizing conditions required to get maximum production. Aspergillus oryzae MTCC 634 was selected and optimized for Tannase enzyme production in solid state fermentation using cheaper sources of sugarcane baggase and rice straw. Tannase production has been evaluated using solid-state fermentation (SSF) at different temperatures, tannic acid, glucose concentration and substrate concentration and incubation time. Addition of tannic acid concentrations increased total activity of crude tannase (27.8 (U/gm/min). Optimum fermentation conditions of pH, temperature and incubation period for Tannase production were found to be 5.5 and 30°C at 72 h. In purification step, 60% ammonium sulphate fractionation 51.6 U/gm/min was found and maximum tannase activity of DEAE Sephadex colum chromatography purified sample was found to be 116.4 U/gm/min. Thus the present study proved that the fungal strainAspergillus oryzae used is highly potential and useful for industrial productions of Tannase. Key words: Tannase, tannic acid, fermentation, purification, rice straw powder and sugarcane baggase.