Occurrence and resistance of bacteria isolated from the rabbit nares - a retrospective evaluation

Abstract

Rabbit snuffles is one of the most common challenges in veterinary practice. The aim of the present study was to evaluate nasal samples of rabbits submitted between 2015-2019, with regard to bacterial distribution and antimicrobial resistance. Each sample was plated on four different agar plates and enriched in a non-selective broth. Isolates were identified by MALDI Biotyper® (MBT) (Bruker Daltonik GmbH, Bremen, Germany) and antimicrobial susceptibility was performed by broth microdilution method in accordance with the Clinical and Laboratory Standards Institute (CLSI, Wayne, PA, USA). A total of 1261 samples were evaluated. Among the samples that tested positive (n=941), one bacterial species was detected in 79.1% of the cases, and more than one bacterial species (mixed culture) was found in 20.9% of the cases. A total of 150 species from 14 families were identified. Isolates belonging to the family Pasteurellaceae were identified most frequently, followed by Enterobacteriaceae, Pseudomonadaceae and Staphylococcaceae.A total of 467 antibiograms of the most common pathogens with possible clinical relevance (Pasteurella multocida [14.6%], Pasteurella species [10.0%], Staphylococcus aureus [5.9%], Pseudomonas aeruginosa [5.4%] and Bordetella bronchiseptica [4.8%]) were evaluated. Quinolones showed the highest efficacy and clindamycin the lowest. Furthermore, among S. aureus, MRSA were most frequently detected in 2016 reaching 23.1% of cases. Since the causal bacteria for rabbit snuffles are mostly found in the deeper areas of the nose and the nasal vestibule is often contaminated with ubiquitous and coliform bacteria, it would make sense to take samples from the depth of the nasal cavity, ideally via nasal lavage. Due to the demonstrated pathogen diversity and long-term therapy associated with the disease, bacterial culture and sensitivity testing is recommended as part of the management. In the absence of an antibiogram, enrofloxacin is the drug of first choice due to its favorable resistance pattern and tolerability. However, since quinolones are considered as "critically important" antibiotics, their use should be limited to a minimum.