Abstract
ABSTRACTA total of 600 samples of milk from different species [buffalo (150), cow (150), goat (150), and sheep (150)] were analyzed for aflatoxin M1 (AFM1) contamination using high-performance liquid chromatography and enzyme-linked immunosorbent assay (ELISA) methods. AFM1 contamination was found in buffalo (38.6%), cow (45.3%), goat (33.3%), and sheep (36.6%) milk. The mean value of AFM1 was 0.026 µg L−1 in buffalo, 0.018 µg L−1 in cow, 0.014 µg L−1 in goat, and 0.017 µg L−1 in sheep milk. In all types of milks, the level of AFM1 concentration was higher in milk obtained from urban and semi-urban areas, whereas it was found minimal in milk from rural areas. The results of the analysis of AFM1 level by the ELISA analysis (ng L−1) was observed in 46.5% of all samples. The amount of AFM1 in 16% buffalo, 44% cow, 10% goat, and 12% sheep milk samples was above the maximum tolerance limit accepted by the European Union.
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