Abstract
The aim of this study, was to characterize the extended-spectrum-β-lactamases (ESBLs) producing clinical strains of Escherichia coli isolated between January 2009 and June 2012 from Algerian hospitals and to determine the prevalence of 16S rRNA methylase among them. Sixty-seven ESBL-producers were detected among the 239 isolates included: 52 CTX-M-15-producers, 5 CTX-M-3-producers, 5 CTX-M-1-producers, 2 CTX-M-14-producers, 2 SHV-12-producers and one TEM-167-producer. Among the ESBL–producing strains twelve harbored 16S rRNA methylase genes: 8 rmtB and 4 armA. rmtB was located on a IncFIA plasmid and armA was located either on a IncL/M or a IncFIA plasmid. RmtB-producing isolates were genotypically related and belonged to the sequence type ST 405 whereas ArmA-producing isolates belonged to ST10, ST 167, and ST 117. This first description of 16S rRNA methylases among E. coli in Algerian hospitals pointed out the necessity to establish control measures to avoid their dissemination.
Highlights
The emergence of β-lactam resistance in Escherichia coli which is the most common cause of Gram-negative infections has become a significant health concern, mainly due to the production of extended-spectrum beta-lactamases (ESBLs) (Pitout and Laupland, 2008)
CTX-M, ArmA, and RmtB in E. coli from Algeria in E. coli, in most regions of the world (Cantón and Coque, 2006). Another worrisome feature is the emergence of isolates resistant to all clinically important aminoglycosides related to the production of 16S rRNA methylases
To date eleven 16S rRNA methylases genes have been identified, armA and rmtB being the most frequently described in Enterobacteriaceae (Galimand et al, 2003; Bogaerts et al, 2007; Yamane et al, 2007; Fritsche et al, 2008)
Summary
The emergence of β-lactam resistance in Escherichia coli which is the most common cause of Gram-negative infections has become a significant health concern, mainly due to the production of extended-spectrum beta-lactamases (ESBLs) (Pitout and Laupland, 2008). CTX-M, ArmA, and RmtB in E. coli from Algeria in E. coli, in most regions of the world (Cantón and Coque, 2006) Another worrisome feature is the emergence of isolates resistant to all clinically important aminoglycosides related to the production of 16S rRNA methylases. To date eleven 16S rRNA methylases genes (rmtA, rmtB, rmtC, rmtD, rmtD2, rmtE, rmtF, rmtG, rmtH, armA, and npmA) have been identified, armA and rmtB being the most frequently described in Enterobacteriaceae (Galimand et al, 2003; Bogaerts et al, 2007; Yamane et al, 2007; Fritsche et al, 2008). As these genes are usually located on plasmids they can transfer to other bacteria (Nagasawa et al, 2014)
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