Abstract
Four Murciano‐Granadina (M‐G) bucks were used to study the effect of season (autumn and spring) on semen motility kinematic parameters. Ejaculates (n = 31/season) were collected twice weekly with an artificial vagina and diluted with Tris‐based extender (1 : 10). Average path velocity (VAP) and linearity (LIN) were evaluated using a Computer‐Assisted Semen Analysis (CASA system). A FASTCLUS procedure was applied to separate spermatozoa into subpopulations based on their motility characteristics. The mean values of both motility kinematic parameters were significantly (p < 0.05) higher in spring than in autumn. Four different motile sperm subpopulations (SP) were identified. In autumn, SP 1 (with a frequency of 17.1%) showed a VAP of 41.5 μm/s and a LIN of 38.3%. SP 2 (37.2%) a VAP of 70.2 μm/s and a LIN of 46.1% SP 3 (19.1%) a VAP of 93.3 μm/s and a LIN of 31.6%. Finally, SP 4 (26.6%) a VAP of 111.8 μm/s and a LIN of 67.2%. In spring, SP 1 (21.1%) a VAP of 46.3 μm/s and a LIN of 50.1%. SP 2 (39.6%) a VAP of 77.0 μm/s and a LIN of 68.6%. SP 3 (17%) a VAP of 87.8 μm/s and a LIN of 40.2%. Finally, SP 4 (22.3%) a VAP of 112.3 μm/s and a LIN of 70.4%. In conclusion, the season of ejaculate collection has a significant effect on sperm motility kinematic parameters of M‐G bucks.
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