Abstract
Precise characterization of biomolecular information such as molecular structures or intermolecular interactions provides essential mechanistic insights into the understanding of biochemical processes. As the resolution of imaging-based measurement techniques improves, so does the quantity of molecular information obtained using these methodologies. DNA (deoxyribonucleic acid) molecule have been used to build a variety of structures and dynamic devices on the nanoscale over the past 20 years, which has provided an accessible platform to manipulate molecules and resolve molecular information with unprecedented precision. In this review, we summarize recent progress related to obtaining precise molecular information using DNA nanotechnology. After a brief introduction to the development and features of structural and dynamic DNA nanotechnology, we outline some of the promising applications of DNA nanotechnology in structural biochemistry and in molecular biophysics. In particular, we highlight the use of DNA nanotechnology in determination of protein structures, protein–protein interactions, and molecular force.
Highlights
Living systems are organic integrations of biomolecules and are controlled by the interactions thereof between each other
Researchers are able to reconstruct the structures of biomolecules such as proteins at an atomic-level resolution [2,5], and accurately investigate intermolecular interactions such as protein–protein interactions (PPIs) [6,7] and protein–DNA interactions [8]
The DNA molecule is the genetic information carrier for living organisms. It is a building block [11,12,13,14,15,16,17,18,19,20,21] for constructing various nanostructures that are summarized as the significant field of DNA nanotechnology [22,23,24,25]
Summary
Living systems are organic integrations of biomolecules and are controlled by the interactions thereof between each other. The development of single-molecule techniques such as single-particle cryo-electron microscopy (cryo-EM) [1,2], single-molecule fluorescence resonance energy transfer (FRET) [3], and single-molecule force spectroscopy [4] has expanded the ability to precisely study single biomolecules. Utilizing these singlemolecule techniques, researchers are able to reconstruct the structures of biomolecules such as proteins at an atomic-level resolution [2,5], and accurately investigate intermolecular interactions such as protein–protein interactions (PPIs) [6,7] and protein–DNA interactions [8]. We anticipate that the interpretation and summary will shed light on more related applications of obtaining precise molecular information via DNA nanotechnology
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