Obtaining potato microtubers in a liquid nutrient medium

Abstract

Obtaining virus-free potato seed material in Kazakhstan is mainly carried out using the method of isolation of apical meristems with the subsequent production of test-tube plants from them on an agar nutrient medium and the further formation of minitubers in greenhouse. Obtaining microtubers from meristem plants in production of potato seed material in vitro is not carried out, although this method is one of the most successful methods for increasing the potato material in vitro . Based on the above issue, we’ve set a goal to optimize the MS liquid nutrient medium for obtaining potato microtubers in vitro from meristem plants with the prospect of introducing this procedure into the process of obtaining seed material at seed farms. The paper presents new data applicable at the initial stages of potato seed production, for production of the first tuberous generation of healthy material, in particular, for replication of plants in vitro and production of microtubers. The results are presented on analysis of morphometric data of plant development in vitro obtained at cultivation of single-bud potato cuttings on ten variants of the Murashige-Skuga liquid medium with various combinations of sucrose concentrations (20 and 30 g/l), kinetin (0,2 and 4 mg/l), gibberellic acid (0, 0,5 and 1 mg/l) in 220 ml glass vessels. The perfect liquid nutrient medium for obtaining developed potato plants in vitro for further production of microtubers has been defined. Efficiency of liquid nutrient medium for obtaining potato microtubers has been determined when growing plants in vitro USING media with various combinations of concentrations of sucrose (60 and 90 g/l), kinetin (0 and 2 mg/l) and 6-benzylaminopurine (0 and 5 mg/l). The results of the study can form the basis for cultivation of plants in a bioreactor with the purpose of mass production of virus-free potato microtubers.