Abstract
We discuss the observation of an unstained single biological macromolecule of human serum low density lipoprotein in a scanning transmission electron microscope (STEM). Using a carbon substrate with 16 Å average thickness and an electron dose of 19 electrons/Å 2 the image obtained from the unstained molecule shows a similar surface substructure as that seen in a negatively stained conventional transmission electron microscope (CTEM) micrograph.
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