Abstract
The protective effect of oak wood and chestnut wood extracts on the gut barrier function of a porcine intestinal epithelial cell line (IPEC-J2) was investigated. Tannins, polyphenolic compounds, are likely to improve the impaired epithelial barrier function by inhibiting the disruptive effect of TNF-α [Ren et al., 2012]. Therefore, extracts from oak and chestnut wood were prepared and tested in an in vitro cell culture model, using non-transformed, differentiated IPEC-J2. Cells were seeded in non-coated Transwell® membrane inserts (1.12 cm2; 0.4 µm pore size) for 7 days, allowing the formation of tight junctions (TJs) and the build-up of a representative intestinal layer, which was observed by an increase of transepithelial electrical resistance (TEER). Cells were pre-treated with a previously selected non-toxic concentration of the respective extracts. On day 8, a stressor was additionally added to the pre-treated cells and to the control. TEER was measured after 24, 48, and 72h. Following the final TEER measurement, a neutral red cytotoxicity assay was performed.
Published Version
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