Abstract
Methods Functional properties of freshly isolated myeloid dendritic cells were analyzed in mixed lymphocyte reactions. Surface expression of costimulatory molecules and myelomonocytic receptors was analyzed by multiparameter flow cytometry. Targeted si-RNA mediated gene knockout was used to determine the impact of specific functional effects mediated by regulatory myelomonocytic receptors. Assays were performed in elite controllers (n=11) and HIV-1 progressors (n=11) or healthy HIV-1 uninfected persons (n=12) as reference cohorts.
Highlights
AIDS Vaccine 2009 Anna Laura Ross Meeting abstracts – A single PDF containing all abstracts in this Supplement is available here. http://www.biomedcentral.com/content/pdf/1471-2105-10-S12-info.pdf
The receptor ILT2 and ILT5 were significantly higher expressed on mDC in controllers, as opposed to either progressors of HIV-1 negative persons
Si-RNA mediated knock out of ILT2 or ILT5 led to significant reduction of the functional activity of dendritic cells, indicating that it has a critical stimulatory effect on these cells
Summary
Unique stimulatory properties of myeloid dendritic cells in individuals with "elite" HIV-1 control. Address: 1Amgen Inc, Seattle, WA, USA and 2Massachusetts General Hospital, Boston, MA, USA * Corresponding author from AIDS Vaccine 2009 Paris, France. Published: 22 October 2009 Retrovirology 2009, 6(Suppl 3):O24 doi:10.1186/1742-4690-6-S3-O24. AIDS Vaccine 2009 Anna Laura Ross Meeting abstracts – A single PDF containing all abstracts in this Supplement is available here. http://www.biomedcentral.com/content/pdf/1471-2105-10-S12-info.pdf
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