O1-20-3 - Circular RNA profile identifies circOSBPL10 is unregulated and as a proliferative factor in gastric cancer

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Background: Circular RNAs (circRNAs), a new star of the non-coding RNA network, have been identified as critical regulators in various cancers. There is increasing evidence that circRNAs represent a class of widespread and diverse endogenous RNAs that may regulate gene expression. Here, we aimed to determine the circRNA expression profile and investigate the functional and prognostic significance of circRNA in GC. Materials and Methods: We investigated the expression profile of circRNAs in three GC samples and paired adjacent normal tissues using ribo-minus RNA sequencing and a bioinformatics analysis. Furthermore, quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to identify circRNA candidates. Molecular and cellular techniques were used to explore the biological function and mechanism of circRNA in GC cells. The prognostic significance was analyzed using the Kaplan-Meier method and the Cox proportional hazards model. Results: We first characterized circular RNA transcripts using RNA-seq analysis of ribosomal RNA-depleted total RNA from three paired normal and cancerous gastric tissues. In all, 15623 distinct circRNA candidates were found in these tissues and at least 5500 distinct circRNAs are differently expressed in GC tissues compared with matched normal tissues. We further characterized one abundant circRNA derived from the OSBPL10 gene, termed circOSBPL10. The expression of circOSBPL10 is often upregulated in GC tissues and the silencing of circOSBPL10 significantly inhibits gastric cancer cell growth, invasion and migration. Furthermore, the level of circOSBPL10 was observed as an independent prognostic marker for overall survival and disease-free survival of patients with GC. Conclusions: Our study revealed the circular RNA profile of GC tissues and characterized a differentially expressed circRNA derived from the OSBPL10 gene. CircOSBPL10 may serve as a new proliferative factor and prognostic marker in gastric cancer.