Nutritional Requirements for Growth of Agaricus brasiliensis

Eustáquio Souza Dias,Pascoal José Gaspar Júnior,Márcia Mayumi Tomizawa,Rosane Freitas Schwan,Danny Lee Rinker

doi:10.4025/actascibiolsci.v33i1.7010

Eustáquio Souza Dias, Pascoal José Gaspar Júnior + Show 3 more

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https://doi.org/10.4025/actascibiolsci.v33i1.7010

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Abstract

The nutritional requirements of A. brasiliensis in culture media were assessed by supplementing a basal medium (g L -1 ): (glucose, 10, KH2PO4, 1, MgSO4.7H2O, 0.5, (NH4)2SO4, 1, pH 5.5) with CaCl2, trace elements (FeSO4.7H2O; MnCl2.4H2O; ZnSO4.7H2O; CuSO4.5H2O), casein, yeast extract, peptone, B-vitamins or amino acids. Evaluations were based on the mycelial growth in solid or liquid culture (mm day -1 or mg day -1 ) and visual analysis of the colony. The addition of CaCl2 and trace elements was very important for the major mycelial growth of the fungi. The addition of casein and inositol to the medium did not have a significant effect on growth. The best growth result in solid medium was obtained with the basal medium plus the addition of yeast extract and peptone. In relation to the other nutrient sources, the mycelial growth in the presence of amino acids darkened the medium after two weeks. The addition of B-vitamins to the basal medium lead to slower mycelial growth; however, growth was more visually dense when compared to other nutritional sources. B-vitamins added separately did not have the same result, suggesting that the fungus requires two or more vitamins at the same time for better mycelial growth.

Highlights

Agaricus brasiliensis [Agaricus blazei Murrill](WASSER et al, 2002) is a mushroom which grows naturally in the mountainous areas near the city of Piedade, São Paulo State, Brazil
It is known as the almond portobello, sun mushroom or Royal Sun Agaricus (SOUZA DIAS et al, 2004), princess mushroom and Himematsutake (ITOH et al, 1994)
Preparatory to large scale composting and production trials using agro-industrial wastes this study evaluates the effect of different nutrients on the growth of A. brasiliensis in liquid and solid culture medium

Summary

Introduction

(WASSER et al, 2002) is a mushroom which grows naturally in the mountainous areas near the city of Piedade, São Paulo State, Brazil. It is known as the almond portobello, sun mushroom or Royal Sun Agaricus (SOUZA DIAS et al, 2004), princess mushroom and Himematsutake (ITOH et al, 1994). Several studies have demonstrated different polysaccharides and other substances from A. brasiliensis with anti-tumor and anti-mutagenic activity The fruit body induction is not a well understood process (KÜES; LIU, 2000; MAGAE et al, 2005). Fruit body induction is influenced by different

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