Nutrition care and exercise: An effect on muscle and fat metabolism in dialysed and transplant patients with obesity

Abstract

s / Nutrition 30 (2014) 1222–1224 1224 response in the concentration range from LOQ to 55 ng for CH and LOQ to 114 ng for 7-OCH. The response factors (in relation to 5-alpha-cholestane) were 0.206 and 0.142 for CH and for 7-OCH, respectively. System and method precisions were sufficient, repeatability of retention time was less than 1 %, repeatability of injection was less than 15%; the repeatability of the whole procedure for cholesterol was 15.8%. Conclusion: The method of SPE separation of cholesterol and its oxidized products form fat matrices followed by GC-MS was adopted and optimized and working characteristics were evaluated. The method is precise, linear and sufficiently sensitive to determine oxysterols in both food and biological materials. Our future work will be focus on applying this method to the evaluation of oxysterols in fish, meat and vegetable dishes created under different types of culinary conditions (frying, baking, roasting). NUTRITION CARE AND EXERCISE: AN EFFECT ON MUSCLE AND FAT METABOLISM IN DIALYSED AND TRANSPLANT PATIENTS WITH OBESITY V. Teplan , I. Kr alov a-Lesn a , A. Mahrov a , M. H ajek , J. Racek , M. Stollov a 1 Dept of Nephrol, Transplant Centre, Inst Clin Exp Medicine, Prague Cardiovascular research centre, Prague, Czech Republic 3 Faculty of Physical Educ and Sport, Charles Univ Prague, Czech Republic Dept of Magnetic Resonance Imaging,Ist Clin Exper Medicine, Prague Dept of Biochem, Charles Univ Pilsen, Czech Republic Rationale: Vascular lesions during long-term dialysis pose a major limitation for organ transplantation. Endothelial progenitor cells (EPC) play important role in the integrity of vascular endothelium and may be integrated into endothelial cells. Recently, there have been studies documenting the beneficial effect of regular physical activity and nutrition on EPC count and onmuscle and fat metabolism in dialyzed and renal transplant patients. The aim of prospective randomized study was to assess the relationship between peripheral EPC, plasma asymmetric dimethylarginine (ADMA), adipocytokines, visceral fat and fat in muscle mass examined by magnetic resonance spectroscopy (MRS) in dialysed (pre-transplant) and transplant patients before and after exercise training and nutritional management. Methods: We monitored 65 patients before (on dialysis) and 6 months after first cadaveric kidney transplantation (Group I). Intervention involved controlled exercise regimen (Rikli Jones, HRQOL SF36-Bref) and nutritional care. Control Group II consisted of 63 non-exercising transplant patients with standard diet matched in age, BMI, HLA typisation, dialysis management, history of cardiovascular disease and immunosuppressive regimen. Nutritional management in Group I consisted of modified medium protein and energy diet (0.8-1.0g/kg and 35 kcal/kg iBW/day supplemented with mixture of keto,hydroxyand L-amino acids/100mg/kg iBW/day). EPC count in peripheral blood, laboratory examination and MRS were performed before and at month 6 after kidney transplantation. Results: After six months period, significant differences were found in EPC, ADMA, adiponectin and visfatin, (ttest, ANOVA all p < 0.01) in Group I. MRS examinations confirmed reducing of extracellular muscle fat from abdominal and calf muscle area after exercise training (p<0.01).This effect was not found in patients with body/fat weight gain.Renal function was stabilized during follow-up and all patients with rejection were excluded. Conclusion: Regular exercise training and nutritional care were associated with increase of EPC and adiponectin, decrease of ADMA and reducing of fat in muscles in non-obese patients examined by MRS in Group I. This regimen may decrease the vascular risk after organ transplantation and remarkable influence patient s well-being. The study was supported by Project MZO NT 13139-3/2012 awarded by the Internal Grant Agency of the Czech Republic.