Abstract
This study reports the start-up, acclimation, and performance of an acidogenesis process as a biological pretreatment method to solubilize Agave bagasse fibers using the native microbiota. The effects of nutrients were evaluated on acidogenesis performance (volatile fatty acid [VFA] production; soluble chemical oxygen demand [sCOD], and VFA yield) in a reactor R(+) supplemented with urea, phosphate salts, and calcium ions and contrasted with a control reactor R(-) containing distilled water. The native microbial lasted ca. 70 days to stabilize the primary technological parameters. Under stable conditions, R(+) improved VFA production by 5 times (101 ± 12 mgCOD/g), sCOD by 1.5 times (137 ± 33 mgsCOD/g), and VFA yield by 45 times (634 ± 54 mgCOD/gremoved) compared with R(-). In R(+) nutrient supplementation promoted an alkaline pH due to urea hydrolysis and the major VFA were acetic acid > butyric acid > and propionic acid. In contrast, in R(-) the pH acidified rapidly and the major VFA were acetic acid > butyric acid > and lactic acid. In R(+) thrived Cellulomonas, Gordonia, and Pseudoclavibacter. In contrast, in R(-) pH became rapidly acidic promoting the growth of several bacteria such as Lactobacillus and Beijerinckia that correlated positively with acid lactic production. In both reactors, Caproiciproduces was linked to the production of VFA acetate, propionate, and butyrate. Findings demonstrate that the native microbiota of Agave bagasse fibers contained members that depending on the nutrient supplementation solubilized the fibers towards the VFA production with different compositions and yield.
Published Version
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