Abstract
The transcriptional terminator tI generates the 3'end of the integrase ( int) gene transcript that is read from the λ PI promoter in lambda phage. We have studied the factors that affect transcription termination in vitro and in vivo at the λ tI terminator. In vitro transcriptional studies showed that tI is about 80% efficient in the presence of purified NusA protein, whereas it is only about 50% efficient in its absence. In vivo studies, where the readthrough transcript of λ tI was measured by quantitative dot blot analysis, gave about 80% efficiency in wild-type strains, but only 60% in the nusA1 mutant strain at non-permissive temperatures. These results support the idea that termination at λ tI in vivo involves interaction with the NusA factor.
Published Version
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