Abstract
Data is given on extraction of an additional number of fibroblast colony forming cells from mouse bone marrow tissue by trypsinization, over and above the number released by mechanical disaggregation of the hematopoietic tissue, and on the effect of feeder cells on the efficiency of fibroblast colony formation (EFCF). Feeder cells were added to some of the cultures after explanation: suspensions of bone marrow or spleen cells for guinea pigs or mice, irradiated in a dose of 60 Hz (/sup 60/Co). The effect of additional feeder on the efficiency of colony formation was determined in experiments with explanation of suspensions of bone marrow cells with the addition of different numbers of feeder cells. It is found that by trypsinization and the use of an irradiated feeder it is possible to increase EFCF for mouse bone marrow and substantially by comparison with EFCF values achieved previously.
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