Nuôi thành thục noãn bò thu nhận từ nang noãn hình thành xoang giai đoạn sớm trong điều kiện in vitro

Abstract

Bovine preantral follicles (0.5–1 mm diameter) were collected from ovaries of slaughtered cows and cultured in vitro in TCM199 medium with 10% fetal bovine serum (FBS). Follicles were cultured growing in 4-well plates, coated mineral oil with the three treatments: (1) TCM199, 10% FBS (control); (2) TCM199, 10% FBS, sodium pyruvate (0.1 mg/mL); (3) TCM199, 10% FBS, sodium pyruvate (0.1 mg/mL), estradiol (1 μg/mL), follicle stimulating hormone (FSH 0.02 UI/mL) and lutenizing hormone (LH 0.01 UI/mL). After 18 days of growing, collect oocytes and make it mature within 24 hours. Criteria were assessed by increasing the size of the follicle and the possible formation of the first terminal. The study results show that in vitro cultures early antral follicles (diameter