Abstract
The nucleotide sequence of the left end of bacteriophage φ29 DNA has been determined. Together with data reported earlier (Yoshikawa et al., 1981), this sequencing comprises the major early genetic region of this viral genome (5708 bp). Computer analysis of the DNA sequences revealed that there are up to fifteen open reading frames which could encode polypeptides containing more than thirty amino acids. The DNA sequence also revealed a number of potential regulatory signals, promoters and ribosome binding sites. The initiation and the termination of transcription and probable early gene products are discussed.
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