Nucleotide sequence of satellite tobacco ringspot virus RNA and its relationship to multimeric forms

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Tobacco ringspot virus, a member of the nepovirus group, supports the increase and encapsidation of coinoculated satellite tobacco ringspot virus RNA (STobRV RNA). The nucleotide sequence of the unit length STobRV RNA, found here to be 359 nucleotide residues for the budblight strain, occurs also in multimeric, repetitive sequence forms. These are able to undergo an autolytic processing reaction to generate biologically active, unit length STobRV RNA (G. A. Prody, J. T. Bakos, J. M. Buzayan, I. R. Schneider, and G. Bruening,1984, In “Abstracts of the 3rd Cold Spring Harbor RNA Processing Meeting, May 16–20,1984,” p. 8). We determined the nucleotide sequence of the monomeric STobRV RNA by combining results from partial enzymatic digestions of the RNA, partial chemical cleavage of cDNA transcribed from the RNA, and analyses of cDNA clones. Other analyses gave the terminal residues of monomeric STobRV RNA: a cytosine-2′:3′-cyclic phosphodiester and a 5′ terminal adenosine. The terminal residues of monomeric RNA and their adjacent nucleotide sequences are consistent with the sequence in the junction region of dimeric RNA, derived from transcripts and cDNA clones, and with the formation of two monomeric STobRV RNAs upon autolysis of dimer, without the gain or loss of a nucleotide residue.