A nucleotide sequence rearrangement distinguishes two isolates of satellite tobacco ringspot virus RNA

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Several strains of tobacco ringspot virus (TobRV) support the replication and encapsidation of satellite tobacco ringspot virus RNA (STobRV RNA). We have compared the nucleotide sequences of four STobRV RNAs, each initially associated with a different isolate of TobRV. A STobRV RNA from a geranium isolate of TobRV and STobRV RNA from the previously analyzed budblight isolate (J. M. Buzayan, W. L. Gerlach, G. Bruening, P. Keese, and A. R. Gould, 1986, Virology 151, 186–199) differed by a single nucleotide residue substitution. STobRV RNAs from TobRV isolates 62L and NC-87 have the same 360-residue nucleotide sequence. This sequence differs from that of the 359-nucleotide residue budblight STobRV RNA principally at locations 100 through 140. The differences between the two sequences in this region are consistent with a rearrangement of blocks of nucleotide residues. The two sequences can be folded with similar patterns of base pairing. All four STobRV RNAs share a sequence of eighty 5′-terminal and of twenty 3′-terminal residues, including the 5′ hydroxyl group and 2′:3′-cyclic phosphodiester group.