Abstract
α-amanitin is a selective RNA polymerase II inhibitor. Treatment of pachytene spermatocytes with this drug leads to the fragmentation of the nucleoli. This fragmentation proceeds more slowly in the pachytene spermatocytes than in the Sertoli cells. Autoradiographic evidence indicates that the extranucleolar HnRNA synthesis is blocked in the pachytene spermatocytes by α-amanitin whereas nucleolar RNA synthesis remains unaffected. Even a completely fragmented nucleolus in a pachytene spermatocyte still synthesizes RNA. In Sertoli cells both the extranucleolar and the nucleolar RNA synthesis is blocked by α-amanitin. These results are discussed and it is suggested that rRNA synthesis in pachytene spermatocytes in some respects differs from that in somatic cells.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
