Abstract
The advent of nucleic acid-based pathogen detection methods offers increased sensitivity and specificity over traditional microbiological techniques, driving the development of portable, integrated biosensors. The miniaturization and automation of integrated detection systems presents a significant advantage for rapid, portable field-based testing. In this review, we highlight current developments and directions in nucleic acid-based micro total analysis systems for the detection of bacterial pathogens. Recent progress in the miniaturization of microfluidic processing steps for cell capture, DNA extraction and purification, polymerase chain reaction, and product detection are detailed. Discussions include strategies and challenges for implementation of an integrated portable platform.
Highlights
The rapid, reliable detection of pathogenic bacteria is imperative in many different industries, of which food and agriculture, healthcare, environmental monitoring, and bio-defense are the four main players [1]
A comprehensive literature survey was carried out for this present paper, and due to the immense amount of literature related to pathogenic detection, our study focuses primarily on rapid portable systems for the nucleic acid-based detection of bacterial pathogens
Different strategies have been used in both research and commercial settings to develop nucleic acidbased sensors and lab-on-a-chip systems
Summary
The rapid, reliable detection of pathogenic bacteria is imperative in many different industries, of which food and agriculture, healthcare, environmental monitoring, and bio-defense are the four main players [1]. With recent devastating outbreaks of Salmonella and Escherichia coli in the United States, the food industry is largely concerned with the detection of pathogenic bacteria in agricultural products and processed foods. The presence of pathogenic bacteria can cost the food industry and consumers many millions of dollars every year due to food recalls, and is estimated to cause over 30,000 hospitalizations and over 1,000 deaths each year in the United States [2]. The development of a fast and sensitive platform for the detection of pathogens in human blood and waste samples is required in order to implement a quick and effective response to an outbreak. A comprehensive literature survey was carried out for this present paper, and due to the immense amount of literature related to pathogenic detection, our study focuses primarily on rapid portable systems for the nucleic acid-based detection of bacterial pathogens
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