Abstract
INTRODUCTIONThis protocol describes a useful way to observe the development of embryos, as well as meristems and young primordia developing at the shoot apex, by confocal microscopy after staining the nuclei with propidium iodide. The number of cells can be exactly quantified in a meristem or in young primordia. Because embryonic and meristematic cells are largely filled out by their nuclei, it is convenient to image only the nuclei. This method allows analysis of whole-mount material, which is more easily reconstructed than sectioned material.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
